Inhibition of Ergosterol Biosynthesis by Etaconazole in Ustilago maydis
نویسندگان
چکیده
Fungal Lipids, Ergosterol, Inhibition, Etaconazole, Fungicide The triazole fungicide etaconazole (C G A 64 251) interferes with the ergosterol biosynthesis in Ustilago maydis by inhibiting the C-14 demethylation o f the sterol nucleus. During the late log growth phase o f U. maydis a novel endogenous sterol metabolite (14x-methyl-ergosta-8,24(28)dien-3/?,6a-diol) was discovered and analyzed, which accumulates under the influence o f the fungicide. The structure o f this metabolite points to a hydroxylation-dehydration mechanism for the introduction o f the double bond at C-5 during the ergosterol biosynthesis.
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